| Media - Bile Esculin Agar For the Identification of Bacteria |
Bile Esculin Agar: Click Here to View Images
Bile Esculin Agar is used in the identification of enterococci.
Growth Characteristics:
Bile esculin agar is both selective and differential. This formulation is
selective because it contains bile salts and sodium azide. The bile salts
are inhibitory to gram-positive bacteria other than enterococci, while sodium
azide is inhibitory to gram-negative bacteria. It is also differential, in that it
contains esculin, which when hydrolyzed by enterococci, causes a
blackening of the media.
Typical Formulation per Liter:
Bile Esculin Agar is used in the identification of enterococci.
Growth Characteristics:
Bile esculin agar is both selective and differential. This formulation is
selective because it contains bile salts and sodium azide. The bile salts
are inhibitory to gram-positive bacteria other than enterococci, while sodium
azide is inhibitory to gram-negative bacteria. It is also differential, in that it
contains esculin, which when hydrolyzed by enterococci, causes a
blackening of the media.
Typical Formulation per Liter:
- Pancreatic Digest of Casein 17.0 g
- Peptic Digest of Animal Tissue 3.0 g
- Yeast Extract 5.0 g
- Oxgall 10.0 g
- Sodium Chloride 5.0 g
- Esculin 1.0 g
- Ferric Ammonium Citrate 0.5 g
- Sodium Azide 0.25 g
- Sodium Citrate 1.0 g
- Agar 13.5 g
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